We narrowed to 1,150 results for: maz

1. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

   Plasmid

   #67985

   Purpose

   Cas9 activity reporter (control) with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

   Plasmid

   #67975

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72667

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

   Plasmid

   #65417

   Purpose

   AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Has Service

   AAV8

   Insert

   hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

   Plasmid

   #65418

   Purpose

   AAV packaging vector for CamKIIa driven KOR DREADD expression

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Insert

   CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNTK human MBD3 PGK-Neo-pA flox

   Plasmid

   #52362

   Purpose

   Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OCT4

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUW-Flag-Mbd3 delta1-70

    Plasmid

    #52372

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-70

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px335 Mettl14 sgRNA #2

    Plasmid

    #61514

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gccgctcccggatctcctgc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-KIF5A tail only

    Plasmid

    #166956

    Purpose

    Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (820-1027aa)

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

    Plasmid

    #202762

    Purpose

    Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    NEUROG2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge