We narrowed to 15,882 results for: LEA
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Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-iGlu3Fast
Plasmid#226500PurposeUltrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate releaseDepositorInsertiGluSnFR3 S72T
TagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationS72TPromoterCMVAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
TagsSV40 NLSExpressionYeastPromoterTEF1 promoterAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag7
Plasmid#175533PurposeExpression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag7
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xMARS
Plasmid#205233PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cellsDepositorInserttwo repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
TagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-HA-myc-nuc (w557-1)
Plasmid#20025PurposeGateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)DepositorTypeEmpty backboneUseEntry vectorTagsHA, Myc, and NLSExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-P4Mx1
Plasmid#108121PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
ExpressionMammalianPromoterCMVAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB_p2_TCRA
Plasmid#131034PurposeExpresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptideDepositorArticleAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLBac-Bat-FluA-trimer
Plasmid#229858PurposeInfluenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1
ExpressionInsectAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-nlsGFP-W
Plasmid#126688PurposeConstitutive, ubiquitous, nuclear-localized GFP expression.DepositorInsertnlsGFP
UseLentiviralTags6xHIS and NLSPromoterEF1aLAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-H2B-KikGR
Plasmid#98649PurposeExpresses a nuclear-localized photoconvertible green-to-red fluorescent proteinDepositorInsertKikGR
TagsH2BExpressionMammalianPromoterCMV-IE enhancer chick beta actin promoterAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only