We narrowed to 15,772 results for: LEA

1. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. NES-EGFP-P4Mx1

   Plasmid

   #108121

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCX-H2B-KikGR

   Plasmid

   #98649

   Purpose

   Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

   Insert

   KikGR
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   CMV-IE enhancer chick beta actin promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

4. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Parp14-c010

   Plasmid

   #173100

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   F1208-G1388

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FnR8-10

   Plasmid

   #182569

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PEX-LV

   Plasmid

   #12120

   Depositor

   Inder Verma

   Article

   Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

   Insert

   Matrix Metalloproteinase 2 (Mmp2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   noncatalytic fragment of MMP2 comprised of the fi…

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pBad-UGAcS

   Plasmid

   #172137

   Purpose

   bacteria expression plasmid for a UDP-GlcNAc sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UGAcS
   

   Expression

   Bacterial

   Mutation

   H605Q- please see depositor comments

   Promoter

   araBAD

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETZZtevPP7CP(C68A,C73A)

   Plasmid

   #27548

   Depositor

   Kathleen Collins

   Article

   Hogg et al RNA. 2007 Jun . 13(6):868-80.

   Insert

   Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
   

   Tags

   TEV protease cleavage site, six histidine tag, an…

   Expression

   Bacterial

   Mutation

   changed two cysteine to alanine (C68A,C73A) to in…

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pelBHisTEV

    Plasmid

    #157741

    Purpose

    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Tags

    PelB signal sequence + His10-tag + TEV protease c…

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-TarHom-Tol2-kanaR-cryaa-CFP

    Plasmid

    #74151

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-CFP
    

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-StagRFP

    Plasmid

    #173014

    Purpose

    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    StagRFP
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-mCherry

    Plasmid

    #108880

    Purpose

    Expression of human histone2B fused to mCherry (nuclear marker)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    H2B-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Expression

    Mammalian

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AviTag-SpyCatcher

    Plasmid

    #72326

    Purpose

    Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    AviTag-SpyCatcher
    

    Tags

    N-term His6 tag and TEV cleavage site

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVTU_260_BirA

    Plasmid

    #63682

    Purpose

    Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    BirA
    

    Tags

    6X Histidine and TEV cleavage site

    Expression

    Yeast

    Promoter

    ADH1/yeast

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pMLS253

    Plasmid

    #114271

    Purpose

    SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

    Insert

    Cbr-Unc-119
    

    Use

    CRISPR and Cre/Lox

    Mutation

    *please see depositor comment below

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST14-DoubleCatcher-H-Lock

    Plasmid

    #216285

    Purpose

    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher H-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 5UAS zfSynaptophysin:GFP

    Plasmid

    #74316

    Purpose

    A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

    Depositor

    Martin Meyer, Stephen Smith

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Insert

    synaptophysin
    

    Tags

    EGFP

    Mutation

    Q12H and deletion of F13 (please see depositor co…

    Promoter

    5UAS

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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