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pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

Plasmid

#160567

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-HCN2 [N71/37R]

Plasmid

#177567

Purpose

Mammalian Expression Plasmid of anti-HCN2 (Rat). Derived from hybridoma N71/37.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Hcn2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#182092

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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LeGO-iG-CSF2RB(A455D)

Plasmid

#125751

Purpose

Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

Depositor

Dirk Heckl

Article

Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

Insert

CSF2RB(A455D)

Use

Lentiviral

Mutation

A455D, codon optimized

Promoter

SFFV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#190508

Purpose

Mammalian Expression of Bassoon scFV. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Bassoon (Rattus norvegicus) recombinant scFV (Bsn Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC6 [N490/24R]

Plasmid

#211590

Purpose

Mammalian Expression Plasmid of anti-TrpC6 (Human) IgG2a R-mAb. Derived from hybridoma N490/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC6 (Homo sapiens) recombinant mouse monoclonal antibody. (TRPC6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

Plasmid

#156411

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

Plasmid

#156402

Purpose

Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Gateway cloning

Tags

EYFP

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut1 [N28/9R-rat IgG2a]

Plasmid

#222184

Purpose

Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a6 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SUR1 scFv [N289/16]

Plasmid

#206735

Purpose

Mammalian Expression of SUR1 scFV. Derived from hybridoma N289/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SUR1 (Rattus norvegicus) recombinant scFV (Abcc8 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/36]

Plasmid

#199422

Purpose

Mammalian expression of Ankyrin-G scFv. Derived from hybridoma N106/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingAnkyrin-G (Human) (ANK3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#190562

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pQC hGAB.wt V5-His IRES G418

Plasmid

#110333

Purpose

Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS2 Glutaminase 2 (GLS2 Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

Plasmid

#223167

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Glycine receptor Alpha3 scFv [N424/45]

Plasmid

#206774

Purpose

Mammalian Expression of Glycine receptor Alpha3 scFV. Derived from hybridoma N424/45 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Glycine receptor Alpha3 (Homo sapiens) recombinant scFV (GLRA3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.7 Na+ channel [N68/6R]

Plasmid

#177566

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Mitofusin-1 scFv [N111/24]

Plasmid

#211603

Purpose

Mammalian Expression Plasmid of Mitofusin-1 scFv. Derived from hybridoma N111/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Mitofusin-1 (Rattus norvegicus) recombinant mouse scFv. (Mfn1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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