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Vh-Clover-CC-S2-mRuby2-Vt

Plasmid

#233227

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-M2-mRuby2-Vt

Plasmid

#233231

Purpose

Vinculin FRET tension sensor with coiled-coil M2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-M2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_SlMIR164b

Plasmid

#231153

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting SlMIR164b

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL17_pPromALB_enhALB_FireflyLuc

Plasmid

#220283

Purpose

Firefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL18_pPromALB_enhALB_RenillaLuc

Plasmid

#220284

Purpose

Renilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega)

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CLIPf

Plasmid

#200787

Purpose

For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

CLIPf-HisTag-FLAG

Tags

CLIPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SNAPf

Plasmid

#200788

Purpose

For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

SNAPf-HisTag-FLAG

Tags

SNAPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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JD135

Plasmid

#229491

Purpose

Ef1a promoter driving expression of adenoviral 100k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

100k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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