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FLAG-Atoh1 del3

Plasmid

#96913

Purpose

Murine Atoh1 with amino acids 214-305 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 214-305

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del4

Plasmid

#96914

Purpose

Murine Atoh1 with amino acids 306-347 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 306-347

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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SNRK gRNA (BRDN0001149393)

Plasmid

#76731

Purpose

3rd generation lentiviral gRNA plasmid targeting human SNRK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SNRK (SNRK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001146759)

Plasmid

#75899

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001147686)

Plasmid

#75900

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001148159)

Plasmid

#75901

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001146871)

Plasmid

#75881

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001147500)

Plasmid

#75882

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001162195)

Plasmid

#75883

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001162192)

Plasmid

#75884

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.2-AP-His

Plasmid

#71973

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.2 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.6-AP-His

Plasmid

#71977

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.6 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.5-AP-His

Plasmid

#71976

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.4-AP-His

Plasmid

#71975

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.4 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-wt

Plasmid

#67909

Purpose

Inducible expression of PIAL2 in E.coli

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSEW-Venus-Sec16A-deltaN

Plasmid

#66605

Purpose

Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Mutation

encodes amino acids 1107-1217 of human Sec16A

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
NKP91 GFP (1034)

Plasmid

#62023

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

Tmem41A (TMEM41A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only