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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript Sp5

Plasmid

#16884

Depositor

Laure Bally-Cuif

Article

Tallafuss et al Development. 2001 Oct . 128(20):4021-34.

Insert

sp5 (sp5 Zebrafish)

Expression

Bacterial

Available Since

July 30, 2008

Availability

Academic Institutions and Nonprofits only
PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

Plasmid

#164044

Purpose

Expression of stdMCP-tdTomato in mammalian cells

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

stdMCP-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChR2-mCherry

Plasmid

#58892

Purpose

AAV-mediated expression of ChR2-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has Service

AAV5

Insert

ChR2-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HA.Tetherin

Plasmid

#41068

Depositor

Paul Spearman

Article

Ali et al PLoS One. 2010 Feb 2;5(2):e9005.

Insert

Tetherin (BST2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pKO (Hyg)

Plasmid

#110087

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mTagBFP2

Plasmid

#105011

Purpose

Mitochondrial marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tat101(AD8)-Flag

Plasmid

#115777

Purpose

pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Tat protein (AD8)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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