We narrowed to 14,384 results for: NTS

1. pGL4.23 C2_10

   Plasmid

   #60287

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   LINGO2 inactive enhancer (LINGO2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGL4.23 C2_14

   Plasmid

   #60290

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   EDARADD inactive enhancer (EDARADD Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGL4.23 C3_5

   Plasmid

   #60291

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   WSCD2 enhancer (WSCD2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGL4.23 C3_6

   Plasmid

   #60292

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   GRM4 enhancer (GRM4 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGL4.23 C3_8

   Plasmid

   #60294

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   KIRREL3 enhancer (KIRREL3 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pGL4.23 C3_11

   Plasmid

   #60297

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   CDKAL1 enhancer (CDKAL1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pENTR D-TOPO-C2_10

   Plasmid

   #60250

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   LINGO2 inactive enhancer (LINGO2 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR D-TOPO-C2_14

   Plasmid

   #60253

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   EDARADD inactive enhancer (EDARADD Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR D-TOPO-C3_5

   Plasmid

   #60254

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   WSCD2 enhancer (WSCD2 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9(BB)-T2A-HygR

    Plasmid

    #118153

    Purpose

    SpCas9 expression vector

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    hSpCas9-T2A-HygR
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid

    #223127

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS SARS-CoV-2 BA.4/5 Spike

    Plasmid

    #186031

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4/5 Spike (S )
    

    Expression

    Mammalian

    Mutation

    BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCAGGS SARS-CoV-2 BQ.1.1 Spike

    Plasmid

    #193710

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BQ.1.1 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Nov. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3.3-SARS2-B.1.617.1

    Plasmid

    #172319

    Purpose

    expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.1 strain (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

    Promoter

    CMV

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.3_SARS2_Mu

    Plasmid

    #177578

    Purpose

    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Mu variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.3_SARS2_Lambda

    Plasmid

    #177579

    Purpose

    expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Lambda variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge