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We narrowed to 10,989 results for: T7

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Showing: 10901 - 10920 of 10989 results

  1. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available Since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

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  3. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Promoter
    T7
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

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  10. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid
    #173762
    Purpose
    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173762-plasmid-map-sequence-id-353460

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  11. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid
    #173763
    Purpose
    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173763-plasmid-map-sequence-id-353459

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  12. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid
    #173764
    Purpose
    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173764-plasmid-map-sequence-id-353458

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  13. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  14. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  15. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162686-plasmid-map-sequence-id-321468

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  16. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

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Showing: 10901 - 10920 of 10989 results