164,826 results

1. HC20 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135220

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE7-SB

   Plasmid

   #228869

   Purpose

   PE7 connected to MLH1-SB via 2A sequence

   Depositor

   Sangsu Bae

   Article

   Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

   Insert

   PE7-2A-MLH1-SB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-GalT-mCherry

   Plasmid

   #214271

   Purpose

   Mammalian expression of GalT-mCherry (Golgi marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   GalT(1-82)-mCherry (B4GALT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_BiSSTe10_GCaMP6f

   Plasmid

   #213818

   Purpose

   AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple Ga12

   Plasmid

   #196061

   Purpose

   Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha12-RLuc8 (GNA12 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Stx4-pmCitrine

   Plasmid

   #92422

   Purpose

   Expression of syntaxin-4 C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   Stx4 (Stx4 Rat)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-AMOT p130

   Plasmid

   #32821

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   E5D

   Promoter

   CMV

   Available Since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMPRA2-CMV-Clover

    Plasmid

    #53539

    Purpose

    Fluorescent reporter control for pMPRA2

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

    Plasmid

    #207953

    Purpose

    nuclease inactive control for TRF1FokI WT

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI (D450A)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQP-CiRISB

    Plasmid

    #138978

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Desmoplakin I tension sensor (F40-based)

    Plasmid

    #119186

    Purpose

    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-ABCB10-ChR2-eYFP

    Plasmid

    #206037

    Purpose

    Express ChR2 in mitochondria

    Depositor

    Lufang Zhou

    Article

    Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

    Insert

    ChR2
    

    Use

    Tags

    ABCB10 mitochondrial leading sequence and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Fuw-dCas9-dead Tet1CD-P2A-BFP

    Plasmid

    #108246

    Purpose

    Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-dead Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

    Promoter

    Ubc

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCrlM

    Plasmid

    #122444

    Purpose

    Expresses myc tagged mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFP.R268

    Plasmid

    #138180

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-tetO-EGFP

    Plasmid

    #84041

    Purpose

    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge