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We narrowed to 16,448 results for: GRN

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Showing: 10921 - 10940 of 16448 results

  3. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  4. pDIV495

    Plasmid
    #177702
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177702-plasmid-map-sequence-id-353319

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  8. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid
    #124300
    Purpose
    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    SENP8 (SENP8 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124300-plasmid-map-sequence-id-242478

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  10. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  16. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125518-plasmid-map-sequence-id-248252

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Showing: 10921 - 10940 of 16448 results