We narrowed to 18,503 results for: Met

1. pGREAT9

   Plasmid

   #170897

   Purpose

   GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NtEUt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pG10-90

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGREAT10

   Plasmid

   #170898

   Purpose

   GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pCmYLCV911

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGREAT12

   Plasmid

   #170900

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGREAT13

   Plasmid

   #170901

   Purpose

   GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pAtUBQ10

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGREAT14

   Plasmid

   #170902

   Purpose

   GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pG10-90

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGB1a2+pNOS_Red-F_NOSt

   Plasmid

   #170887

   Purpose

   GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientation

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   Red-F
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   p.S286Y Red mutant

   Promoter

   pNOS

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

   Plasmid

   #136886

   Purpose

   Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   VP64 activator domain
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-anticalin (E143amb)-His

   Plasmid

   #168046

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Glu 143 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-anticalin (S87amb)-His

   Plasmid

   #168044

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ser 87 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-anticalin (I55amb)-His

    Plasmid

    #168042

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ile 55 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C513-E06: attB5r-pA-polyhedrinp>-attB1r

    Plasmid

    #162948

    Purpose

    Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter with upstream polyA sequence
    

    Use

    Synthetic Biology

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid

    #162965

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid

    #162964

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

    Plasmid

    #162957

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

    Plasmid

    #162955

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

    Plasmid

    #162954

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

    Plasmid

    #162934

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge