We narrowed to 88,516 results for: MAL

1. pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis

   Plasmid

   #157486

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA7A (SEMA7A Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDECKO_GFP

   Plasmid

   #141210

   Purpose

   empty GFP vector for cloning sgRNA or pgRNAs

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

   Plasmid

   #139444

   Purpose

   Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   sequence codon-optimized

   Promoter

   SFFV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-EB3-mNeonGreen

   Plasmid

   #196848

   Purpose

   labelling of microtubule plus ends

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EB3-NeonGreen (MAPRE3 Human)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer10b-EGFP-KRAS-WT

   Plasmid

   #164935

   Purpose

   Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Promoter

   Ubc Promoter and Ubc promoter

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2K7bsdUBI-mCherry-STIM1

   Plasmid

   #114178

   Purpose

   Lentiviral vector for expression of mCherry-STIM1

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry (inserted after signal peptide)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-RP-CoV2/RBD-puro

   Plasmid

   #161793

   Purpose

   Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

   Insert

   SARS-CoV-2 RBD
   

   Use

   Transposon

   Tags

   6xHis and dTomato

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

   Plasmid

   #134925

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF3351

   Plasmid

   #144827

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARNTL (BMAL1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid

    #114078

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid

    #134922

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid

    #134924

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid

    #197265

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK Zeo p53DD

    Plasmid

    #136534

    Purpose

    Lentiviral expression vector for an inducible p53DD

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Expression

    Mammalian

    Mutation

    This construct was created from the pBabe p53DD (…

    Available Since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid

    #197264

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid

    #134923

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid

    #201956

    Purpose

    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO.neo_shGFP

    Plasmid

    #110470

    Purpose

    Control, silence GFP gene, doxycycline inducible, neomycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-NCR3-COMP5AP-AviTag-9xHis

    Plasmid

    #157057

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NCR3 (NCR3 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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