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  2. pFsynW SYT1 reporter

    Plasmid
    #197275
    Purpose
    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197275-plasmid-map-sequence-id-389085

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  3. pFsynW SYB2 reporter

    Plasmid
    #197276
    Purpose
    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYB2 and HaloTag (Vamp2 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197276-plasmid-map-sequence-id-389092

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  8. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid
    #140840
    Purpose
    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    Use
    Tags
    CsoS2 N-peptide consensus to CbbL C-terminus and …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140840-plasmid-map-sequence-id-296743

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  10. pFsynW SYT1deltaC2AB reporter

    Plasmid
    #197279
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197279-plasmid-map-sequence-id-389097

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  19. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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Showing: 1081 - 1100 of 1230 results