Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,273 results for: nls

Showing: 1621 - 1650 of 6273 results

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pJM589 EF1α intN-ZF10-NLS-HA in TUPV2

Plasmid

#161565

Purpose

Constitutive expression of intN-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intN-ZF10-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB004 CMV 3xFLAG-NLS-VP16-intN

Plasmid

#161549

Purpose

Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP16-intN

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB005 CMV 3xFLAG-NLS-intC-ZF2

Plasmid

#161550

Purpose

Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-intC-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM516 EF1α intC-ZF1-NLS-HA in TUPV2

Plasmid

#161551

Purpose

Constitutive expression of intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM522 EF1α intC-ZF10-NLS-HA in TUPV2

Plasmid

#161552

Purpose

Constitutive expression of intC-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF10-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB002 CMV 3xFLAG-NLS-DsDed-ZF1

Plasmid

#161532

Purpose

Constitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

Plasmid

#161534

Purpose

Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159941

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE

Plasmid

#159948

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

Plasmid

#159947

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

Plasmid

#159945

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTFP1.0-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-YPET-optoNES-WPRE

Plasmid

#159942

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-mScarlet-optoNES-WPRE

Plasmid

#159943

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mScarlet-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-Dronpa-optoNES-WPRE

Plasmid

#159944

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-Dronpa-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE

Plasmid

#159946

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

Plasmid

#159975

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.F434L

Use

AAV

Expression

Mammalian

Mutation

F434L

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

Plasmid

#159973

Purpose

optical regulation of nuclear export with extended relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.V416I

Use

AAV

Expression

Mammalian

Mutation

V416I

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

Plasmid

#159974

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.I427V

Use

AAV

Expression

Mammalian

Mutation

I427V

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145271

Purpose

Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(-7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat deleted

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145266

Purpose

Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HOXA13-IDR-mCherry-CRY2-NLS

Plasmid

#145265

Purpose

Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

Plasmid

#130197

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-Sirius

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI

Plasmid

#130198

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-SuperNova

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI

Plasmid

#130214

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-Eos

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

Plasmid

#133796

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1 (truncated C-terminal domain only)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

C-terminal domain only

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

Plasmid

#133797

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T16A)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T16A

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

Plasmid

#133799

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Leu-AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

Plasmid

#133800

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Eriv-AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA

Plasmid

#140224

Purpose

Constitutive PGK driven expression of intein with miniVPR for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

C-intein with miniVPR

Tags

mCherry

Expression

Mammalian

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-VALENTIA-030 NLSNoLS-mVenus//BsaI

Plasmid

#130211

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-mVenus

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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