We narrowed to 4,128 results for: pCMV mammalian

1. pCMV5-FLAG-MKK6-K82M

   Plasmid

   #21583

   Depositor

   John Kyriakis

   Article

   Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

   Insert

   MKK6 (MAP2K6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   lysine 82 to methionine

   Available Since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-Tag4A-MMP8 (G104R)

   Plasmid

   #29548

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   G104R

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-Tag4A-MMP8 (E87D)

   Plasmid

   #29547

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   E87D

   Available Since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

   Plasmid

   #185910

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

   Plasmid

   #185915

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-FLEx-TetOn3G-3xHA-FLEx

    Plasmid

    #133729

    Purpose

    Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    TetOn3G-3xHA (floxed & inverted)
    

    Use

    AAV

    Tags

    3xHA-tag

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-sport2-mGCN5 FU, FLAG Tagged

    Plasmid

    #23098

    Depositor

    Sharon Dent

    Article

    Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

    Insert

    mouse Gcn5 mRNA (Kat2a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    missing 50 bp upstream of ATG start site.

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-Flag-YAP-S94/F95A

    Plasmid

    #33057

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S94A, F95A

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-RA-HRas-IRES2-Lyn-iRFP682

    Plasmid

    #180584

    Purpose

    RA-Hras and Lyn-iRFP682

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    RA-Hras and Lyn-iRFP682
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

    Plasmid

    #205275

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    E498R

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

    Plasmid

    #205276

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    T513K

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

    Plasmid

    #205274

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D487K

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

    Plasmid

    #205272

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D300R

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV Lex VP16 (F442A) HA (P#1710)

    Plasmid

    #14595

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Tags

    HA and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCMV Ub Lex VP16 (F442A) HA (P#1711)

    Plasmid

    #14596

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Tags

    HA, Ubiquitin, and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R273H

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R248W

    Available Since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV, lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-Neo-Bam p53 R249S

    Plasmid

    #16438

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R249S (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R249S

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV Fcgamma RIIa IRES neo

    Plasmid

    #14948

    Depositor

    Axel Nohturfft

    Article

    Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Fcgamma RIIa (FCGR2A Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV5-rat ERK2-L73P/S151D

    Plasmid

    #40819

    Depositor

    Natalie Ahn

    Article

    Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L73P, S151D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pCMV-FLAG TIF1gamma M (456-887)

    Plasmid

    #15735

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma M (TRIM33 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Middle region (amino acids 456-887).

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-Flag-YAP-4SA(S109)

    Plasmid

    #33081

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S127A, S164A, S381A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pCMV-Flag-YAP-4SA(S61)

    Plasmid

    #33080

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S109A, S127A, S164A, S381A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

35. 3xFN-Tel-TAL/pCMV-7.1

    Plasmid

    #63589

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-Flag-YAP-4SA(S127)

    Plasmid

    #33082

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S109A, S164A, S381A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pCMV-Flag-YAP-4SA(S164)

    Plasmid

    #33083

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S381A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pCMV-Flag-YAP-S127/384A

    Plasmid

    #33076

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S127A, S384A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pCMV-Flag-YAP-S127/387A

    Plasmid

    #33079

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S127A, S387A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pCMV PhiC31 attB dsRed Hygro

    Plasmid

    #242885

    Purpose

    Selectable donor plasmid for insertion by PhiC31 integrase into pre-inserted attP landing pad site

    Depositor

    Jesse Owens

    Article

    Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

    Insert

    Hygromycin-IRE2S-DsRED Express 2
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge