We narrowed to 7,555 results for: ALP

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]

   Plasmid

   #177486

   Purpose

   Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

   Plasmid

   #122966

   Purpose

   Expresses 3XFlag-tagged human CEP164 in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   CEP164 (CEP164 Human)
   

   Use

   Lentiviral

   Tags

   3XFlag

   Promoter

   EF1 alpha

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Stat1 alpha Y701F Flag pRc/CMV

   Plasmid

   #8702

   Depositor

   Jim Darnell

   Article

   Wen et al Cell 1995 Jul 28;82(2):241-50.

   Insert

   STAT1 alpha Y701F (STAT1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y701F

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. V309 - pDNR MCS SA lacZ alpha - Donor

   Plasmid

   #11692

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Donor vector

   Available Since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PBS/pU6- HIF1 alpha RNAi plasmid 1

   Plasmid

   #21103

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against hypoxia inducible factor 1alpha
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

   Plasmid

   #225433

   Purpose

   Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

   Depositor

   Diego Orzaez

   Insert

   4xCBS:mDFR:Rep/RepA:Tnos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   alpha ENAC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204343

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PBS/pU6-HIF1 alpha RNAi plasmid 2

    Plasmid

    #21104

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    RNAi against hypoxia inducible factor 1alpha
    

    Expression

    Mammalian

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

    Plasmid

    #136603

    Purpose

    Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    FKBP-C-mSOG-J-alpha
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV MP-135 mRFP-3MREs (AAV-alpha)

    Plasmid

    #206929

    Purpose

    Alpha cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-135

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

    Plasmid

    #117662

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT1)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204342

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

    Plasmid

    #122991

    Purpose

    Expresses Flag-tagged hRPGRIP1L in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    RPGRIP1L (RPGRIP1L Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    EF1 alpha

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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