We narrowed to 1,991 results for: CCS

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pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

Plasmid

#126477

Purpose

Expresses Cas9-5xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
5xFlag
TdT (DNTT Human)

Expression

Mammalian

Mutation

Changed codons to reduce repetitive sequences.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL210

Plasmid

#126451

Purpose

Expresses Cas9-XTEN-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G2

Plasmid

#86610

Purpose

Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via nhej using ouabain

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Full Length (pGEX4T1)

Plasmid

#238421

Purpose

Expresses GRP78 Full Length fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

Plasmid

#233389

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rg(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_Cas9-hGem_gRNA2

Plasmid

#217659

Purpose

expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA2 targeting the human AAVS1 safe harbour locus

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB4

Plasmid

#200983

Purpose

Mammalian expression plasmid for myc-tagged EphB4

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB4-Fc

Plasmid

#200986

Purpose

Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB4

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

Plasmid

#191151

Purpose

Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L72:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_p53(R280K)_V5

Plasmid

#136540

Purpose

Lentiviral expression vector for an inducible p53(R280K)-V5

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

V5

Expression

Mammalian

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-Rac1 5'-UTR

Plasmid

#115354

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

Rac1 5'-UTR (RAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-p38 β/MAPK11 5'-UTR

Plasmid

#115357

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

p38 β/MAPK11 5'-UTR (MAPK11 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1346

Plasmid

#119274

Purpose

knockdown folA in K. pneumoniae

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (K. pneumoniae)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1223

Plasmid

#119261

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1221

Plasmid

#119260

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-R2Tg-RTmut

Plasmid

#238106

Purpose

For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

Reverse transcriptase mutant R2Tg, mammalian codon optimized

Use

For ivt

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #1

Plasmid

#174511

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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