We narrowed to 8,833 results for: Pol

1. WT (REP10)

   Plasmid

   #21368

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. PROM_MpUBE2

   Plasmid

   #136109

   Purpose

   PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM_MpUBE2 Mapoly0106s0019
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. 5UTR_MpUBE2

   Plasmid

   #136110

   Purpose

   5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   5UTR_MpUBE2 Mapoly0106s0019
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. WT (AF20)

   Plasmid

   #21359

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wild-type sequence*

   Promoter

   Available since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6 MCV LTco S239A

   Plasmid

   #112194

   Purpose

   Expression of MCPyV LT mutant (Ser 239 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM41-EcLonA

   Plasmid

   #38330

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   Lon protease fragment (lon Escherichia coli)
   

   Use

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Polyphosphate binding fragment of Lon protease. …

   Promoter

   T7

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA6 MCV LTco S147A

   Plasmid

   #112192

   Purpose

   Expression of MCPyV LT mutant (Ser 147 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCV-HF LTS220A/S239A

   Plasmid

   #112191

   Purpose

   Merkel cell polyomavirus molecular clone

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCV-HF LTS220A/S239A
   

   Use

   Tags

   Expression

   Mutation

   T to G mutations at 3313 (nt) and 3370 (nt) of fu…

   Promoter

   None from vector. Insert contains native promoter…

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PROM5_MpU6

    Plasmid

    #136120

    Purpose

    Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpU6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDS12-MpER-Targ

    Plasmid

    #136094

    Purpose

    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    ER targeting peptide from predicted chitinase Mapoly0069s0092
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCRISPRyl

    Plasmid

    #70007

    Purpose

    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2015 Dec 29.

    Inserts

    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-PARP1-Flag-His-WT

    Plasmid

    #111572

    Purpose

    Expresses full length PARP1 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCCM

    Plasmid

    #162708

    Purpose

    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 PBD wt (Nigg AH12)

    Plasmid

    #41162

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603)

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3 PBD HK-AA (Nigg AH13)

    Plasmid

    #41161

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD HK-AA (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLIB-FLAG(N)-RPB1

    Plasmid

    #231722

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    POLR2A (POLR2A Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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