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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Use

Tags

6xHis and V5

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Use

Tags

Expression

Bacterial

Mutation

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (F126A)-HA

Plasmid

#133067

Purpose

Retroviral expression of mouse MD2 (F126A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (F126A) (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

F126A

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C95A

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-SENP8 sg2R-P2A-Hygro

Plasmid

#124300

Purpose

Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

SENP8 (SENP8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1178

Plasmid

#111104

Purpose

Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1224

Plasmid

#111105

Purpose

Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1118

Plasmid

#111110

Purpose

Expresses HELQ_+_84376961.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

HELQ gRNA (HELQ Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1165

Plasmid

#111111

Purpose

Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

HELQ gRNA (HELQ Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1082

Plasmid

#111102

Purpose

Expresses FANCE_-_35420187.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCE gRNA (FANCE Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1135

Plasmid

#111103

Purpose

Expresses FANCE_-_35420147.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCE gRNA (FANCE Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe H-Ras 12V

Plasmid

#12545

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

H-Ras 12V (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V constituitively activated.

Promoter

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone1

Plasmid

#162128

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP13

Plasmid

#159614

Purpose

Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

Depositor

Opher Gileadi

Article

Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

Insert

Covid-SARS2 Nsp13

Use

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

T7 - LacO

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-hOCT3/4-shp53-F

Plasmid

#27077

Purpose

Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

OCT3/4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pCXLE-hUL

Plasmid

#27080

Purpose

Integration-free (episomal) expression of human L-MYC and LIN28

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

L-MYC (LIN28A, MYCL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2011

Availability

Academic Institutions and Nonprofits only

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ATG-2460

Plasmid

#108713

Purpose

EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt-copGFP fusion

Use

Lentiviral

Tags

CBR2opt fused to copGFP

Expression

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

elongation factor-1

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ATG-3925 (CBG2)

Plasmid

#167839

Purpose

Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

Depositor

Lance Encell

Article

Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

Insert

Click Beetle Green 2

Use

Tags

6-His

Expression

Bacterial and Mammalian

Mutation

Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

Promoter

CMV, T7

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-IRES-BSD

Plasmid

#209751

Purpose

Lentiviral transfer plasmid to express the blasticidin resistance gene

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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