CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
Type
Blog Post
... and/or promoters to knock out lncRNA function (Hazan and Bester 2021; Zibitt et al. 2021). Mutating splice...inserted to disrupt the transcription of the ncRNA (Hazan and Bester 2021). For example, inserting a polyadenylation...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.
Alternative CRISPR systems...chromatin structures or other local genomic elements (Hazan and Bester 2021).
Other Cas variants
Another option...337(6096):816-821. doi:10.1126/science.1225829
Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput...