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pAAV-hSyn-IgK-R-eLACCO2.1-COBRA

Plasmid

#223349

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2.1

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET29a-α3-E

Plasmid

#187935

Purpose

Express phage alpha3 lysis protein E in bacteria

Depositor

Yi Ma

Article

Ma et al Bioengineering (Basel). 2022 Sep 2;9(9):

Insert

Phage alpha 3 lysis protein E

Tags

Strep II

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAG Retro EGFP Neo

Plasmid

#234464

Purpose

Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.

Depositor

Melina Fan, Addgene Research Program

Insert

Green Fluorescent Protein

Use

Retroviral

Available Since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MCP/PCP yellow RNA Lantern

Plasmid

#226096

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-CAF1-C-10

Plasmid

#231782

Purpose

Visualization of nucleus in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-CAF1-C-10 (Chaf1a Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNK115

Plasmid

#219745

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal luciferase

Use

Luciferase and Synthetic Biology

Mutation

I3S, N4T, F11L, I63T, T99P, T192S, A199P

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLPE1

Plasmid

#209981

Purpose

Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE2

Plasmid

#209982

Purpose

Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE3

Plasmid

#209985

Purpose

Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_cat)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7-SCOUT14.G

Plasmid

#201002

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT_WPRE

Plasmid

#216414

Purpose

Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc_AraC-S170-LOV

Plasmid

#206805

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

Insert

AraC-AsLOV2 fusion

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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4A3.SU

Plasmid

#197675

Purpose

Expresses P1 S tail fibre and U chaperone

Depositor

Baojun Wang

Article

Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

Inserts

S
U

Expression

Bacterial

Promoter

Late S promoter, LPS and N/A

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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H2B CFP

Plasmid

#198059

Purpose

mCerulean fused to human H2B protein

Depositor

Amro Hamdoun

Insert

H2B

Use

Synthetic Biology; Sea urchin, mammal, zebrafish

Tags

mCerulean

Promoter

sp6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT2MPEF-TOMM-QUE37C

Plasmid

#191526

Purpose

ATP measurement, mitochondria outer membrane, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTorPE-FRISZ

Plasmid

#176888

Purpose

Far-red fluorescent zinc indicator FRISZ in pTorPE vector

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Tags

Periplasmic expression TorA tag and Twin-Strep-ta…

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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