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p7xEGFP-N1

Plasmid

#122167

Purpose

Expression of 7xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

7xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-5xSunTag (SRv1)

Plasmid

#185797

Purpose

pcp-5xSunTag component for SunRISER SRv1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-5xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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p9xEGFP-N1

Plasmid

#122169

Purpose

Expression of 9xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

9xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p11xEGFP-N1

Plasmid

#122171

Purpose

Expression of 11xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

11xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK

Plasmid

#126577

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSP64-H2B-GFP

Plasmid

#135605

Purpose

Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-GFP

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Pax3

Plasmid

#84316

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pax3 (Pax3 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pknox2

Plasmid

#74354

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pknox2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx/knotted 1 homeobox 2 (Pknox2 )

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mED

Plasmid

#35033

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

PT MUTANT E160D

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Six4

Plasmid

#84323

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Six4 (Six4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Plcb2

Plasmid

#105991

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Plcb2 (Plcb2 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx1

Plasmid

#84317

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx1 (Pbx1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJK350

Plasmid

#71560

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Tags

His6

Expression

Bacterial

Mutation

mutation changes alanine-365 to aspartate

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pZLrhaB2plus

Plasmid

#211787

Purpose

pSCrhaB2plus with modifications of resistance cassette to CmR

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

CmR

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBaseline-PRM-wasabi

Plasmid

#195560

Purpose

Always-off, unactivated expression of mWasabi reporter

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Insert

mWasabi

Tags

AANDENYADAS ssrA tag

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_517-938_H

Plasmid

#146459

Purpose

Bacterial Expression of NcQDE2_517-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_517-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MiniSOG-SpAGS-GFP

Plasmid

#185581

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG-SpAGS

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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