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BB1_23_MS2bind_VP64_SV40NLS

Plasmid

#161479

Purpose

BB1 encoding a MS2 binding VP64 fusion protein with an additional SV40 NLS

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Insert

MS2 binding protein + VP64 activation domain

Use

Synthetic Biology

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKM634

Plasmid

#134219

Purpose

Baculovirus expression of CENP-HIKM

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP HIKM

Expression

Insect

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pKM672

Plasmid

#134221

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 1-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-419

Plasmid

#104720

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MPK3 (MPK3 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-22

Plasmid

#104708

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 W40A (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

W40A, BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-131

Plasmid

#104713

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MIEL1 (AT5G18650 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-489

Plasmid

#104715

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

NEDD8 (RUB1 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-65

Plasmid

#104716

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

Exo70B2 (EXO70B2 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-349

Plasmid

#104717

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

FLS2 kinase domain (FLS2 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC33

Plasmid

#104698

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC33 (UBC33 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC34

Plasmid

#104699

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC34 (UBC34 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC35

Plasmid

#104700

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC35 (UBC35 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC36

Plasmid

#104701

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC36 (UBC36 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-135

Plasmid

#104703

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MMZ1/UEV1A (MMZ1 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-136

Plasmid

#104704

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MMZ2/UEV1B (MMZ2 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-137

Plasmid

#104705

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MMZ3/UEV1C (MMZ3 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC20

Plasmid

#104685

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC20 (UBC20 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge