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pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5154

Plasmid

#128704

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

avrPto

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV810

Plasmid

#126603

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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B4GALT1-Dox

Plasmid

#124633

Purpose

Doxycycline-inducible B4GALT1 expression in mammalian cells

Depositor

Ron Weiss

Publication

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Beta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Publication

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Expression

Mammalian

Mutation

Q71L

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pNapin-eGFP

Plasmid

#127797

Purpose

Napin Promoter driven eGFP expression

Depositor

Scott Poethig

Publication

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Expression

Plant

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP21-1A(sfGFP)

Plasmid

#126968

Purpose

Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D(sfGFP)

Plasmid

#126963

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1C(sfGFP)

Plasmid

#126962

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1B(sfGFP)

Plasmid

#126961

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-D387A

Plasmid

#59778

Purpose

Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

Depositor

Won Do Heo

Publication

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-D387A (FGFR1 Human, Mustard Weed)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Publication

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Publication

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-mRFP

Plasmid

#117778

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-TagBFP

Plasmid

#117780

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-eGFP

Plasmid

#117782

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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