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pDMJ2-SARS-CoV-2-Spike (B.1-2a)

Plasmid

#241038

Purpose

Expresses SARS-CoV-2 spike (B.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T478K,R346T,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2b)

Plasmid

#241039

Purpose

Expresses SARS-CoV-2 spike (B.1-2b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1)

Plasmid

#241033

Purpose

Expresses SARS-CoV-2 spike (B.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-BRAF-WT-IRES-mCherry

Plasmid

#221027

Purpose

Fluorescent reporter for expressing a segment of wild type BRAF

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

BRAF wildtype (BRAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAD120-CHIP-G132N-myc-His

Plasmid

#236053

Purpose

Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv [Preprint]. 2025 Feb 17:2025.02.

Insert

CHIP-G132N (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

G132N

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAD000-CHIP-K30A-myc-His

Plasmid

#236052

Purpose

Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv [Preprint]. 2025 Feb 17:2025.02.

Insert

CHIP-K30A (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

K30A

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3317)

Plasmid

#239299

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

Plasmid

#238177

Purpose

Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

HES7 sgRNA CRISPRa (HES7 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW

Plasmid

#234609

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) with all aromatic ami…

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS

Plasmid

#221117

Purpose

FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3-Tailless

Expression

Yeast

Mutation

STE3 mutant that lacks the entire C-terminal tail…

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-shCdkn1a(#4)

Plasmid

#228913

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

shCdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK3-GK-CR

Plasmid

#229424

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK3-GK-CR (MARK3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M132G; silent mutation CRISPR…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-GK-CR

Plasmid

#229423

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-GK-CR (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M129G; silent mutation CRISPR…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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