We narrowed to 22,710 results for: ARC

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fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Src

Plasmid

#19965

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

Src (SRC Chicken)

Expression

Mammalian

Mutation

K295M

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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IDP424

Plasmid

#87018

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

CLIP-Nrx1B-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C2

Plasmid

#141247

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOME0015R

Plasmid

#178385

Purpose

Expresses KSHV ORF45

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 45

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

Plasmid

#69903

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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MD~E-pCLBabe

Plasmid

#20912

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD~E12 forced dimer

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Short flexible linker region between the MyoD and…

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 S208A

Plasmid

#27115

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S208A

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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[GFP][IRES][Bla]

Plasmid

#97194

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR (GFP) cRel shRNA1

Plasmid

#22509

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA1 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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