171,009 results

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U6-5'+3' MS2-CircRNA

Plasmid

#213752

Purpose

For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV RT∆RNase H

Plasmid

#213749

Purpose

For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot1 (CNOT1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR10-YFP

Plasmid

#13643

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 10 (TLR10 Human)

Tags

YFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur

Plasmid

#60523

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PA-GFP-α-Tubulin

Plasmid

#213900

Purpose

Imaging of Tubulin using Photoactivatable GFP

Depositor

Lukas Kapitein

Insert

PA-GFP-α-Tubulin

Tags

Photoactivatable GFP

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-CDT2

Plasmid

#215091

Purpose

Expresses FLAG-CDT2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDT2 (DTL Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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LXRalpha_pcDNA3.1

Plasmid

#177621

Purpose

Full-length human LXR alpha cDNA sequence in pcDNA3.1+

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

LXR alpha (NR1H3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBZCas13b

Plasmid

#89898

Purpose

Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Fp16SrRNA

Plasmid

#218519

Purpose

pUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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