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pUt-mNF-BACH1

Plasmid

#199219

Purpose

LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

hTetR::P2A::EGFP::BACH1 (BACH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV D2ir promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GID4-Nluc

Plasmid

#200879

Purpose

Mammalian expression of human GID4 with C-terminal NanoLuciferase fusion

Depositor

Stefan Knapp

Article

Owens et al Nat Chem Biol. 2024 Sep;20(9):1164-1175.

Insert

GID4-NanoLuciferase (C17orf39 Human)

Expression

Mammalian

Mutation

WT

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hAP1 420-440

Plasmid

#183643

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only the trans…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7(res)

Plasmid

#160106

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.E190K

Plasmid

#81704

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

E190K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.R897Q

Plasmid

#81714

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

I655V; R897Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.K6N

Plasmid

#81698

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

K6N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.F90S

Plasmid

#81693

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

F90S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.P33S

Plasmid

#81676

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

P33S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G127V

Plasmid

#81683

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

G127V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G129V

Plasmid

#81685

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

G129V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G127R

Plasmid

#81650

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

G127R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.R233Q

Plasmid

#81655

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

R233Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WBSCR17_p.E507K

Plasmid

#81619

Purpose

Gateway Donor vector containing WBSCR17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WBSCR17 (GALNT17 Human)

Use

Gateway entry vector

Mutation

E507K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_p.E510K

Plasmid

#81601

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

E510K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WBSCR17_p.D348N

Plasmid

#81579

Purpose

Gateway Donor vector containing WBSCR17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WBSCR17 (GALNT17 Human)

Use

Gateway entry vector

Mutation

D348N; R375L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_p.R194C

Plasmid

#81573

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

R194C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WBSCR17_p.E289K

Plasmid

#81575

Purpose

Gateway Donor vector containing WBSCR17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WBSCR17 (GALNT17 Human)

Use

Gateway entry vector

Mutation

E289K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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