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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-EGFP-L

Plasmid

#52480

Purpose

Expresses mCherry and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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p35ST

Plasmid

#51514

Purpose

pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

T30 TALEN pair
us:NPTII repair template

Use

Plant expression t-dna

Tags

NLS and flag

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD20-GFP

Plasmid

#80651

Purpose

Promoter1 with BCD20-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD21-GFP

Plasmid

#80652

Purpose

Promoter1 with BCD21-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P279L

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NG

Plasmid

#90420

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NG

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NN

Plasmid

#90419

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NN

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NI

Plasmid

#90418

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NI

Plasmid

#90422

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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p35SZ.D

Plasmid

#51512

Purpose

pMDC32 with Zif268:FokI followed by us:NPTII repair template sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant expression t-dna

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pJJH1308

Plasmid

#36911

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-XRE-dUnaG-PEST-Myc

Plasmid

#252287

Purpose

Reporter for Ahr activity

Depositor

Roland Friedel

Inserts

Xenobiotic Response Elements (XREs) core motif promoter
dUnaG-PEST-degron

Use

Lentiviral

Tags

c-myc tag

Promoter

XRE (Xenobiotic response element 5X + CMV minimal…

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGS1T-PylRSC6a-PylTm15CUA

Plasmid

#251554

Purpose

Production of engineered aaRS/tRNA pair for C6a incorporation, also suitable for C5a and C7a incorporation

Depositor

Alexandria Deliz Liang

Article

Deliz Liang et al Unpublished

Inserts

PylRS-C6a
PylT-m15_CUA

Expression

Bacterial

Promoter

Glutamine synthetase promoter (GlnS) and proK

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pGS1T-PylRSC6a-MmtRNAPylCUA

Plasmid

#251555

Purpose

Production of engineered aaRS/tRNA pair for C6a incorporation with lower yield but low background in the absence of C6a

Depositor

Alexandria Deliz Liang

Article

Deliz Liang et al Unpublished

Inserts

PylRS-C6a
MmtRNA-Pyl_CUA

Expression

Bacterial

Promoter

Glutamine synthetase promoter (GlnS) and proK

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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S’RP + PodJ’

Plasmid

#241365

Purpose

SpmX△TM-mRFP-PopZ, sfGFP-PodJ249pse

Depositor

Hsiao-Chun Huang

Article

Chou et al Commun Biol. 2025 Jul 20;8(1):1078. doi:

Inserts

SpmX△TM-mRFP-PopZ
sfGFP-PodJ249pse

Expression

Bacterial

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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altAncNeo

Plasmid

#196469

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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AncBird

Plasmid

#196450

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2025.07.04.662692

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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