We narrowed to 11,688 results for: nts

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MAC-C Sars-CoV-2 NSP13_E341D

Plasmid

#194814

Purpose

Sars-CoV-2 NSP13_E341D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_E341D (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

E341D

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP13_P77L

Plasmid

#194815

Purpose

Sars-CoV-2 NSP13_P77L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_P77L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P77L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP2_T85I

Plasmid

#194816

Purpose

Sars-CoV-2 NSP2_T85I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP2_T85I (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T85I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_A890D

Plasmid

#194817

Purpose

Sars-CoV-2 NSP3_A890D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_A890D (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

A890D

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_K977Q

Plasmid

#194818

Purpose

Sars-CoV-2 NSP3_K977Q in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_K977Q (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

K977Q

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PX458-3xHA-FnCas9ABEmax8.17d

Plasmid

#201955

Purpose

Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

Plasmid

#202058

Purpose

Inducible AAV with reporter gene and cloning site to add activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-noActivator Domain -Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITRs intact

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_PWP1:1-501

Plasmid

#211189

Purpose

Mammalian expression of full-length human PWP1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PWP1:M1-S501 (PWP1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_PWP1:1-501

Plasmid

#211262

Purpose

Mammalian expression of full-length human PWP1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PWP1:M1-S501 (PWP1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#184717

Purpose

iRaf

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCOA-Leu-FvPPT1

Plasmid

#179923

Purpose

Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

FvPPT1

Expression

Yeast

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Yarrowia lipolytica EXP1

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA-R206H

Plasmid

#206182

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S194A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA-R206H

Plasmid

#206183

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S192A, S194A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-VSAS-R206H

Plasmid

#206185

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

T189V, S192A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-VASS-R206H

Plasmid

#206186

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

T189V, S190A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-In18runon-PTPN22

Plasmid

#195377

Purpose

A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Gateway Cloning

Mutation

Intron 18-runon PTPN22

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

Plasmid

#146212

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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