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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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RAB14 S25N

Plasmid

#61495

Purpose

to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Mutation

S25N, inactive form

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc

Plasmid

#84656

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
p8389 LentiCRISPRv2 Neo sgNT-1

Plasmid

#221649

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-1

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRS263

Plasmid

#122379

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW110031

Plasmid

#222437

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p5193 pGL4-LCR-HPV16-LCR (nt 7000-100)-luciferase

Plasmid

#32888

Depositor

Peter Howley

Article

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Insert

LCR HPV16

Promoter

LCR

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHB-2

Plasmid

#140955

Purpose

For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Mutation

R238S mutation in PhaC

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37

Plasmid

#196657

Purpose

Plasmid for overexpression of native initiator tRNA (A37)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37 expression cassette

Expression

Bacterial

Promoter

metY native promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP GFP

Plasmid

#45238

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, GFP

Tags

GFP and full length CENP-B

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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B2395_CIBN-BTB-IDR-FRB

Plasmid

#215107

Purpose

Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CIBN-BTB-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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RSV-betaGal

Plasmid

#24058

Depositor

Herbert Samuels, Frederick Stanley

Article

Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

Insert

LacZ (lacZ E. coli)

Tags

RSV promoter

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
GFP-TCL

Plasmid

#23231

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

TCL (RHOJ Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pUPD2+pEstrInd

Plasmid

#170880

Purpose

Phytobrick - Estradiol Inducible Promoter

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pEstradiol_Inducible

Expression

Plant

Mutation

c.837C>T and c.1353G>A to remove BsaI and E…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry2-mEGFP

Plasmid

#127816

Purpose

Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry2-mEGFP

Expression

Mammalian

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-APC11

Plasmid

#19900

Depositor

Yue Xiong

Article

Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

Insert

APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pBS_hNFATc4nuc

Plasmid

#24586

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

NFATc4 (NFATC4 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S167A, S169A, S172A, S173A, S174A, S213A, S217A, …

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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