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AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

Plasmid

#191709

Purpose

AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-(-2)AP-Ub

Plasmid

#232582

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Publication

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_RFP

Plasmid

#206177

Purpose

mammalian expression of RFP

Depositor

Christopher Goodnow

Publication

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RFP

Expression

Mammalian

Mutation

NA

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-CFP-Venus(H148G)

Plasmid

#47501

Purpose

Fluorescent reporter for pH inside primary cilia

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus(H148G)

Tags

5HT6 and CFP

Expression

Mammalian

Mutation

H148G mutation

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCGT-Sos Cat

Plasmid

#32923

Depositor

Dafna Bar-Sagi

Publication

Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SOS1 (SOS1 Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Contains aa 564-1049 (catalytic domain)

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
CS-TRE-BMI1-Ubc-rtTA

Plasmid

#198191

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Publication

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hBMI1 (BMI1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Publication

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Publication

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEMS1375

Plasmid

#29176

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ple88 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR-Myc-IKKalpha

Plasmid

#19739

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IkB kinase alpha (Chuk Mouse)

Tags

Myc

Expression

Mammalian

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

Plasmid

#223747

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Publication

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Lambda-PPase

Tags

6xHis-TEV

Expression

Bacterial

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TevC-IRES-SP6-Cre

Plasmid

#171620

Purpose

M13-TevC with Cre

Depositor

Hyungbae Kwon

Publication

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

M13-TevC-IRES-SP6-Cre

Use

AAV and Synthetic Biology

Tags

6x His

Expression

Mammalian

Promoter

hSyn

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCIG PIK3CA E545K

Plasmid

#73055

Purpose

Human PIK3CA E545K

Depositor

Joseph Gleeson

Publication

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIK3CA (PIK3CA Human)

Expression

Mammalian

Mutation

E545K

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP11(x7)-Actin

Plasmid

#181967

Purpose

Human beta actin tagged with 7 copies of GFP11.

Depositor

Jin Zhang

Publication

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP11(x7)-Actin (ACTB )

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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