We narrowed to 11,628 results for: 110

1. pmCherry-imp α

   Plasmid

   #119719

   Purpose

   Expresses mCherry-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

   Insert

   importinα (KPNA2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   contains amino acids 251-529

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-N-Myc_535-888 USP7

   Plasmid

   #131248

   Purpose

   Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 EG.5.1

   Plasmid

   #212454

   Purpose

   Encodes SARS-CoV-2 variant EG.5.1 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike EG.5.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-PIK3CG

   Plasmid

   #116774

   Purpose

   Lentiviral expression of PIK3CG

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CG (PIK3CG Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

   Plasmid

   #108693

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A-D34A/D169A
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon, …

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-N-Myc_535-775 USP7

   Plasmid

   #131247

   Purpose

   Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. STT3A pEGFP-N2 (1051)

   Plasmid

   #62025

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.5

   Plasmid

   #212523

   Purpose

   Encodes SARS-CoV-2 variant XBB.1.5 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike XBB.1.5 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225703

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K36M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   K36M

   Promoter

   pEF1A

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Spike-deltaC18 XBB

    Plasmid

    #212521

    Purpose

    Encodes SARS-CoV-2 variant XBB Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Jam2-Fc-His

    Plasmid

    #72079

    Purpose

    Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam2 (Jam2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid

    #145770

    Purpose

    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Stop codon at Serine 2013

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pSS02:cyt-Peredox-mCherry_DS

    Plasmid

    #161747

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry_DS
    

    Expression

    Plant

    Mutation

    amino acid substitution D115S in first T-Rex doma…

    Promoter

    Ubiquitin 10

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90213

    Purpose

    To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Human, Mouse)
    

    Use

    Retroviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

    Plasmid

    #212538

    Purpose

    Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-PIK3CB-E1051K

    Plasmid

    #116553

    Purpose

    Lentiviral expression of PIK3CB E1051K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Lentiviral

    Mutation

    E1051K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-PIK3CG-E267K

    Plasmid

    #116577

    Purpose

    Lentiviral expression of PIK3CG E267K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Lentiviral

    Mutation

    E267K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

    Plasmid

    #223148

    Purpose

    Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310

    Promoter

    EF1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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