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pMRE-Tn7-137

Plasmid

#118555

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0060

Plasmid

#172783

Purpose

Level 0 module containing NT2_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0061

Plasmid

#172784

Purpose

Level 0 module containing CT_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-ancestral-retroCHMP3-HA

Plasmid

#154248

Purpose

expresses ancestral New World monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed ancestral sequence of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mNeonGreen (ErmR)

Plasmid

#177208

Purpose

Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

Depositor

Alexey Merz

Insert

mNeonGreen

Expression

Bacterial

Mutation

Yeast codon optimized

Promoter

Synthetic constitutive promoter

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA

Plasmid

#62929

Purpose

pLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

0.5synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

Plasmid

#62930

Purpose

pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.1Synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK253

Plasmid

#173750

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Phsp-16.48::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Phsp-16.48

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pmRuby2-IRES-Clover

Plasmid

#171059

Purpose

Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

IRES

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-TDP-43 M337V

Plasmid

#162609

Purpose

Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E23: attB5r-mRuby3-nostop-attB1r

Plasmid

#162945

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E22: attB5r-mClover3-nostop-attB1r

Plasmid

#162944

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT1R1AtR2NtB2

Plasmid

#160885

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T1

Expression

Bacterial

Promoter

rbcS-T1: T7, chaperones: T8

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS5R1AtR2NtB2

Plasmid

#160884

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-S5

Expression

Bacterial

Promoter

rbcS-S5: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM8

Plasmid

#133671

Purpose

Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SNF7-GFP (SNF7 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R_Keima

Plasmid

#127146

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R

Plasmid

#127135

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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