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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MultiMate-PE2 HEK3

Plasmid

#206274

Purpose

Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP

Use

CRISPR; Recombinant baculovirus production, cre-a…

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 1

Plasmid

#198489

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#1/Cre

Plasmid

#193231

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#1 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#2/Cre

Plasmid

#193232

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#2 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgMroh2b#2/Cre

Plasmid

#193220

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#2 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgMroh2b#1/Cre

Plasmid

#193219

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#1 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-1

Plasmid

#184476

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT2-2

Plasmid

#184483

Purpose

Lentivirus gRNA targeting human LIPT2 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT2 (LIPT2 Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-2

Plasmid

#184477

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT2-1

Plasmid

#184482

Purpose

Lentivirus gRNA targeting human LIPT2 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT2 (LIPT2 Human)

Use

Lentiviral

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRi

Plasmid

#134989

Purpose

dCas9-mediated inactivation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

Plasmid

#165085

Purpose

gRNA 1 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

Plasmid

#165086

Purpose

gRNA 2 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Gpi1

Plasmid

#175934

Purpose

knockout mouse Gpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gpi1 gRNA (Gpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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