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We narrowed to 10,790 results for: T7

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Showing: 2201 - 2240 of 10790 results

  1. 4JXT B

    Plasmid
    #60106
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RPRD1B:DCC020-G07:C222611 (RPRD2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. p(+)MV-NSE-FlagrP-3g_haP-F497D-3p

    Plasmid
    #58800
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497D gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag and Ha
    Promoter
    T7
    Available Since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58800-plasmid-map-sequence-id-91095

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  3. HOXD4-WV-MSCV

    Plasmid
    #21002
    Depositor
    Corey Largman
    Insert
    HOXD4 (Hoxd4 Mouse)
    Use
    Retroviral
    Tags
    His and T7-tag
    Expression
    Mammalian
    Mutation
    W->V PBX1 interaction mutant
    Available Since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only

  4. pRSF:His6-CylA-27-412

    Plasmid
    #241275
    Purpose
    For expression of the Enterococcal cytolysin activating protease CylA
    Depositor
    Wilfred van der Donk
    Insert
    CylA protease
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N-terminal secretion signal sequence removed (res…
    Promoter
    T7
    Available Since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241275-plasmid-map-sequence-id-487216

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  5. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

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  7. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  8. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  9. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  10. pET28a_HCK

    Plasmid
    #224239
    Purpose
    Expression of human HCK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224239-plasmid-map-sequence-id-459258

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  11. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

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  12. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  13. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid
    #218198
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM
    Depositor
    Florence Pojer
    Insert
    EndoCOM
    Tags
    3C-CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 19-315
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218198-plasmid-map-sequence-id-431794

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  14. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

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  15. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212984-plasmid-map-sequence-id-420357

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  16. pT7CFE1_EVD68_3ABC

    Plasmid
    #212979
    Purpose
    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212979-plasmid-map-sequence-id-420356

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  17. pT7CFE1_EVD68_3ABCD

    Plasmid
    #212981
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212981-plasmid-map-sequence-id-420354

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  18. pGEMHE-TNKS-2-M1054V

    Plasmid
    #138538
    Purpose
    For making TNKS-2-M1054V RNA
    Depositor
    Carmen Williams
    Insert
    Tankyrase 2 (TNKS2 Human)
    Use
    In vitro transcription
    Tags
    FLAG
    Mutation
    Changed methionine 1054 to valine
    Promoter
    T7
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138538-plasmid-map-sequence-id-291736

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  19. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  20. pmCellFree_KA1_SARS-COV-2_N

    Plasmid
    #169401
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _N (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169401-plasmid-map-sequence-id-336866

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  21. pmCellFree_KA1_SARS-COV-2_ORF6

    Plasmid
    #169392
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169392-plasmid-map-sequence-id-335430

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  22. 6WAT

    Plasmid
    #162268
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF1 (PHF1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162268-plasmid-map-sequence-id-319831

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  23. 6PED

    Plasmid
    #162265
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    HEMK2 (N6AMT1 Human)
    Tags
    6xHis-Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162265-plasmid-map-sequence-id-319828

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  24. TBXTA-c025

    Plasmid
    #139762
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139762-plasmid-map-sequence-id-296575

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  25. TBXTA-c024

    Plasmid
    #139761
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139761-plasmid-map-sequence-id-296544

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  26. TBXTA-c023

    Plasmid
    #139760
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139760-plasmid-map-sequence-id-296543

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  27. 6CW0 (cgd2_2690)

    Plasmid
    #119965
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_2690
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1-105
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119965-plasmid-map-sequence-id-232689

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  28. STK10 (STK10A-c043)

    Plasmid
    #110255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK10 (STK10 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110255-plasmid-map-sequence-id-212331

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  29. pT7-7 asyn S129W

    Plasmid
    #105744
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129W (SNCA Human)
    Expression
    Bacterial
    Mutation
    S129W
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105744-plasmid-map-sequence-id-207297

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  30. pT7-7 asyn K32R

    Plasmid
    #105739
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein K32R (SNCA Human)
    Expression
    Bacterial
    Mutation
    K32R
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105739-plasmid-map-sequence-id-207290

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  31. pET11a asyn Q79N (TAC-->TAT)

    Plasmid
    #105733
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    Expression
    Bacterial
    Mutation
    Q79N
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105733-plasmid-map-sequence-id-207258

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  32. 5J3U (TgPKA, 583.m05422)

    Plasmid
    #79525
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    583.m05422
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79525-plasmid-map-sequence-id-150111

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  33. 5FEA (LdBRD19, LdBPK_363520.1)

    Plasmid
    #79520
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363520.1
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79520-plasmid-map-sequence-id-150104

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  34. 5CZG (Tb427.10.7420)

    Plasmid
    #79517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427.10.7420
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79517-plasmid-map-sequence-id-150098

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  35. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

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  36. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

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  37. pJK292

    Plasmid
    #73371
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changed histidine-59 to asparagine and histidine-…
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73371-plasmid-map-sequence-id-135107

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  38. pJK293

    Plasmid
    #73372
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changed histidine-59 to phenylalanine and histidi…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73372-plasmid-map-sequence-id-135108

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  39. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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  40. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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Showing: 2201 - 2240 of 10790 results