We narrowed to 1,991 results for: CCS
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Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mRuby3-6xFLAG
Plasmid#161613PurposeCell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.DepositorInsertmRuby3-6xFLAG
UseAAVExpressionMammalianMutationN/APromoterHuman synapsin 1 promoterAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N192Q/N200Q
Plasmid#121453Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN192Q/N219QPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1 3SA
Plasmid#121493PurposeExpression of human PD-L1 3SA in mammalian cellsDepositorAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-T453D
Plasmid#235209PurposeExpresses GRP78 with T453D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationChange Threonine 453 to Aspartic AcidAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-G227D
Plasmid#235207PurposeExpresses GRP78 with G227D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationChange Glycine 227 to Aspartic AcidAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRW398-URA3-BXB1-LP
Plasmid#235977PurposePlasmids for generation of donor DNA for construction of a BXB1-compatible landing pad p1 with URA3 selection marker.DepositorInsertURA3
Available SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export
Plasmid#233388PurposePiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as markerDepositorInsertIL2Rb(ecto+TM)-ADAR2dd
TagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorInsertSMC4-mAID-HaloTag (SMC4 Human)
ExpressionBacterial and MammalianAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-C1-Nir1-LNS2
Plasmid#220085Purposehigh affinity PA biosensor with far-red fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
TagsiRFP670-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-BirA*-HA
Plasmid#214474PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.DepositorInsertBirA(R118G)
UseGateway Cloning and LentiviralTagsHA tag and Lyn11 (Plasma Membrane Localization Se…ExpressionMammalianMutationR118GPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
ExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)
Plasmid#191177PurposeMammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).DepositorInsertmCherry:GAPDH.s1:EGFP (frameshifted)
ExpressionMammalianMutationEGFP is frameshiftedPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)
Plasmid#191147PurposeExpression of the sequence matching the 54 extra bases in EK0362 in mammalian cellsDepositorInsertBdfn.t+54
ExpressionMammalianMutationWTPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT miRc2 3xFLAG
Plasmid#83274PurposeEmpty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAGDepositorTypeEmpty backboneUseGateway CloningTags3xFLAGPromoterTRE tightAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GDF11-V5 blast
Plasmid#83098PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only