We narrowed to 10,911 results for: NSI

1. CMV-CAR-GECO1

   Plasmid

   #45493

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRRL-VinculinTS-A50I

   Plasmid

   #111831

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I) (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed vinculin alanine 50 to isoleucine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EFF-1::V5

   Plasmid

   #132961

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   EFF-1::V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXWJ109Hg-gfp

   Plasmid

   #123148

   Purpose

   A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, PmerT

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-N1-mt-ro1GFP

   Plasmid

   #82407

   Purpose

   Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Use

   Tags

   leader sequence of the E1α subunit of pyruvate de…

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBabe-PI(WT)-Src(WT)-mCherry

   Plasmid

   #87356

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src (Src Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-PI(WT)-Vav2(DPZ)

   Plasmid

   #86974

   Purpose

   PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   VAV2 DH/PH/C1 (Vav2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE30-roGFP1-iL

   Plasmid

   #83313

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iL
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

   Promoter

   T5

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCLX-M38s/TOP-dGFP

   Plasmid

   #114295

   Purpose

   Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   destabilized GFP

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ORF57 Pr pGL4.16

    Plasmid

    #120378

    Purpose

    Firefly luciferase reporter with KSHV ORF57 early gene promoter

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF57 Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    ORF57 Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUE1-TSP-ORF68

    Plasmid

    #162650

    Purpose

    Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMZ-180

    Plasmid

    #195428

    Purpose

    Transient, constitutive expression of synZiFTR: ZF5-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMZ-181

    Plasmid

    #195429

    Purpose

    Transient, constitutive expression of synZiFTR: ZF6-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF6-4 (ZF6) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Yellow Cameleon-Nano30/pcDNA3

    Plasmid

    #51963

    Purpose

    mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCAGGS::AtHAP2-YFP

    Plasmid

    #132960

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    At HAP2::YFP
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ORF31-2xStrep pCDNA4

    Plasmid

    #129744

    Purpose

    Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMZ-177

    Plasmid

    #195425

    Purpose

    Transient, constitutive expression of synZiFTR: ZF2-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF2-6 (ZF2) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge