We narrowed to 10,938 results for: NSI;

1. pMC-1-WDV-LRS-L-11

   Plasmid

   #197720

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   WDV LRSL in c-sense with GG expansion to v-sense ORF position
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCiVSP(C363S)-SD64TF

   Plasmid

   #80334

   Purpose

   Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

   Depositor

   Yasushi Okamura

   Article

   Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

   Insert

   Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Changed Cysteine 363 to Serine

   Promoter

   SP6

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yellow Cameleon-Nano15/pcDNA3

   Plasmid

   #51961

   Purpose

   mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. PylRS-AS

   Plasmid

   #137908

   Purpose

   For incorporation of fluorophenylalanine derivatives

   Depositor

   Wenshe Liu

   Article

   Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Containing N346A, C348S mutations

   Promoter

   pBAD

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-VinculinTS-I997A

   Plasmid

   #111832

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A) (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed vinculin isoleucine 997 to alanine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-1-WDV-LS-L-11

   Plasmid

   #197719

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   WDV LSL in c-sense with GG expansion to v-sense ORF position
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMZ-178

   Plasmid

   #195426

   Purpose

   Transient, constitutive expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMZ-185

   Plasmid

   #195433

   Purpose

   Transient, constitutive expression of synZiFTR: ZF10-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV-CAR-GECO1

    Plasmid

    #45493

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL-VinculinTS-A50I

    Plasmid

    #111831

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFF-1::V5

    Plasmid

    #132961

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    EFF-1::V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-mt-ro1GFP

    Plasmid

    #82407

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/T65S/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-PI(WT)-Vav2(DPZ)

    Plasmid

    #86974

    Purpose

    PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/C1 (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30-roGFP1-iL

    Plasmid

    #83313

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iL
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

    Promoter

    T5

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCLX-M38s/TOP-dGFP

    Plasmid

    #114295

    Purpose

    Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    dGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    destabilized GFP

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. ORF57 Pr pGL4.16

    Plasmid

    #120378

    Purpose

    Firefly luciferase reporter with KSHV ORF57 early gene promoter

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF57 Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    ORF57 Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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