We narrowed to 49,092 results for: prot

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRH2961

Plasmid

#210144

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET16b-RPA4/His14 (MSW#949)

Plasmid

#208075

Purpose

Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4, RPA14(His-tagged)

Tags

10xHis tag on RPA14

Expression

Bacterial

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713-P2A-GFP

Plasmid

#197172

Purpose

Expresses the protein of mRhubarb713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubard713-P2A-GFP

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2-P2A-GFP

Plasmid

#197155

Purpose

Expresses the protein of miRFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2-P2A-GFP

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT025-24 Angstrom 12TMD de novo_mRFP1_3x FLAG

Plasmid

#202507

Purpose

Express 24 Angstrom 12TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

24 Angstrom 12TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT026-32 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202508

Purpose

Express 32 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

32 Angstrom 4TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT028-40 Angstrom 12TMD de novo_mRFP1_3x FLAG

Plasmid

#202510

Purpose

Express 40 Angstrom 12TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

40 Angstrom 12TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT024-24 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202506

Purpose

Express 24 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

24 Angstrom 4TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT027-40 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202509

Purpose

Express 40 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

40 Angstrom 4TMD de novo

Tags

mRFP1-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget_PS_VA

Plasmid

#186454

Purpose

pTarget containing protospacer for FnCas12a targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget_PS_VB

Plasmid

#186456

Purpose

pTarget containing protospacer for AaCas12b targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmGIGYF-dsRNAres_AH

Plasmid

#148928

Purpose

Insect Expression of DmGIGYF1-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-dsRNAres (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF-Y58AY60AM65A_AH

Plasmid

#148907

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1B

Plasmid

#198335

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1A

Plasmid

#198334

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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