161,641 results

1. pMMB67EH-dddAI

   Plasmid

   #186559

   Purpose

   Broad host range regulated expression of the DddA immunity determinant (DddAI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRC-CMV-Rev1b

   Plasmid

   #204153

   Purpose

   Lentivirus helper plasmid coding for Rev

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCX-SpiCee

   Plasmid

   #140836

   Purpose

   mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. (+)-Limonene Synthase

   Plasmid

   #112230

   Purpose

   (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

   Depositor

   Daniel Oprian

   Article

   Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

   Insert

   (+)-Limonene Synthase
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-52

   Promoter

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167937

   Purpose

   Inducible CRISPRa expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   dxCas9(3.7)-VPR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-NLS-HA-Dre

   Plasmid

   #51272

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Dre
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAF0033 Ef1a-Pa01-2a-EGFP

   Plasmid

   #193460

   Purpose

   Expression of Pa01 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

   Plasmid

   #98927

   Purpose

   Cre dependent AAV expression of GFP spaghetti monster myc construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Has Service

   AAV1

   Insert

   GFP-smFP-myc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-BRD3

   Plasmid

   #65377

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD3 (BRD3 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Clover

    Plasmid

    #40259

    Purpose

    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge