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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
ELF2_pet28a

Plasmid

#131662

Purpose

Bacterial expression of ETS gene ELF2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF2 (ELF2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L95E

Plasmid

#47344

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pBABE hygro MEN1 L22R

Plasmid

#11021

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 L22R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

L22R

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pmiRGLO-BAX-201

Plasmid

#117332

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR BAX-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 136X

Plasmid

#11049

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 136X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
GFP SDPR

Plasmid

#68398

Purpose

Expression of fluorescently tagged SDPR in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SDPR (Sdpr Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
MSCV N-term HA-FLAG puro BRD7 shRescue

Plasmid

#41936

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-N

Plasmid

#27418

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus of ZO-2, so only…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKsub(TA)DD-YPet-NES

Plasmid

#84634

Purpose

Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsub(TA)DD-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC101

Plasmid

#62335

Purpose

sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB2b

Plasmid

#19470

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2b (DNAJB2 Human)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep164

Plasmid

#136824

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep164 (CEP164 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-KIAA0841

Plasmid

#136810

Purpose

Mammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-KIAA0841 (HAUS5 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDESTmycEIF2C1

Plasmid

#19871

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only