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pEntry_HCoV-HKU1-nsp6

Plasmid

#168895

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp6 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp8

Plasmid

#168897

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp8 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp5

Plasmid

#168868

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp5 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp6

Plasmid

#168869

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp6 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp9

Plasmid

#168872

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp9 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp3

Plasmid

#168838

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp3 (orf1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp1

Plasmid

#168841

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp1 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp8

Plasmid

#168850

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp8 (ORF1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp7

Plasmid

#168820

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp7 (orf1ab )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Publication

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCK760

Plasmid

#192643

Purpose

Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

Depositor

Jesse Zalatan

Publication

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23110-sfGFP

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23110

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5392-CRISPRai-HIS3

Plasmid

#182831

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HIS3 marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5393-CRISPRai-TRP1

Plasmid

#182832

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5391-CRISPRai-LEU2

Plasmid

#182830

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-Q11

Plasmid

#185790

Purpose

Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Publication

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-Q11

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Publication

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_247

Plasmid

#180529

Purpose

Entry vector containing pTac promoter

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pTac promoter

Expression

Bacterial

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-sfGFP-GMRWhite

Plasmid

#165907

Purpose

Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pG418R-MCS-QF-MiniWhite

Plasmid

#165902

Purpose

G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

Plasmid

#137859

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Expression

Mammalian

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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