We narrowed to 63,974 results for: cell

1. pCMV 3xFLAG-GABARAPL2 G116A

   Plasmid

   #123104

   Purpose

   Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMP926

   Plasmid

   #135001

   Purpose

   m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG, Dam(R95A), and DpnI(aa146-254)

   Expression

   Mammalian

   Mutation

   DpnI domain truncated to aa146-254. Dam protein m…

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV 3xFLAG-GABARAPL2 G116

   Plasmid

   #123105

   Purpose

   Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-ECFP-GIT1

   Plasmid

   #15223

   Depositor

   Rick Horwitz

   Article

   Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847

   Insert

   G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
   

   Tags

   ECFP and flag

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pGL3-U6-pegRNA_SRD5A3-EGFP

   Plasmid

   #159781

   Purpose

   S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMED5-bio-His

   Plasmid

   #53338

   Purpose

   Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TMED5 (TMED5 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST40-2XFL-Src-Y419F

   Plasmid

   #140317

   Purpose

   Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y419F

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-FHH-MTOR HEAT

   Plasmid

   #120709

   Purpose

   Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MTOR HEAT 1-9 (MTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Amino acids 1-409

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-ING1a

    Plasmid

    #79069

    Purpose

    Expression of p47ING1 in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

    Insert

    ING1 (p47 ING1A isoform) (ING1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pGS_N_3xFLAG

    Plasmid

    #101654

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, 3xFLAG tag
    

    Tags

    3xFLAG tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCDNA3-N3FLAG-MYB-FL

    Plasmid

    #105609

    Purpose

    transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Full length MYB (mouse) (Myb Mouse)
    

    Tags

    3*FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBI-CMV1-mir144[909 nt]~451-CMV2-mir1

    Plasmid

    #164681

    Purpose

    Express miR-144[909 nt]~451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144[909 nt]~451 (MIR144 Human)
    miR-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVPS13D-I-EGFP

    Plasmid

    #176464

    Purpose

    express internally GFP-tagged VPS13D in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    EYFP is internally inserted

    Promoter

    CMV

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV Gab1(PRD)-mCherry-HOTag3

    Plasmid

    #190026

    Purpose

    Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    Gab1(PRD) CluMPS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP-RAPH1-F1

    Plasmid

    #194860

    Purpose

    Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-535

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Full human ELL2

    Plasmid

    #127253

    Purpose

    Expresses full-length human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. A2M-bio-His

    Plasmid

    #53423

    Purpose

    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    A2M (A2M Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LB1366 (Bcl-2 promoter with MHS1234)

    Plasmid

    #15388

    Depositor

    Linda Boxer

    Article

    Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Bcl-2 full promoter with IgH full enhancer MHS1234

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pFX-β-tubulin-Venus

    Plasmid

    #175828

    Purpose

    Express Venus with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with microtuble-targeting sequence
    

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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