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pcDNA5-FRT/TO-FLAG-SHLD1

Plasmid

#114115

Purpose

Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_CDC42_p.Q61L

Plasmid

#82174

Purpose

Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDC42 (CDC42 Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTCF_WT

Plasmid

#81789

Purpose

Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTCF (CTCF Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.T80K

Plasmid

#81511

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

T80K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway entry vector

Mutation

E545K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.G31A

Plasmid

#81524

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

G31A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAF1_WT

Plasmid

#82317

Purpose

Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAF1 (RAF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-ER-KLF2-T2A-mCHERRY

Plasmid

#224297

Purpose

Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Estrogen Receptor

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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px330-Pten-2

Plasmid

#162552

Purpose

sgRNA targeting Pten

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Pten (Pten Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_p.R24C

Plasmid

#82175

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway entry vector

Mutation

R24C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DNMT3A_WT

Plasmid

#81765

Purpose

Gateway Donor vector containing DNMT3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNMT3A (DNMT3A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DVL3_WT

Plasmid

#82267

Purpose

Gateway Donor vector containing DVL3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DVL3 (DVL3 Human)

Use

Gateway entry vector

Mutation

W433L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hMer

Plasmid

#225996

Purpose

Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PGL4-4X TE Down (GQs) Renilla

Plasmid

#70039

Purpose

Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

4x TE Down (G-quadruplexes) in 5'UTR

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Mutation

4 repeats of TE down motif (G-quadruplexes) in 5&…

Promoter

SV40

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_CHD2_WT

Plasmid

#81768

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TCF7L2_WT

Plasmid

#81769

Purpose

Gateway Donor vector containing TCF7L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PER1_WT

Plasmid

#82152

Purpose

Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PER1 (PER1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PAK1_WT

Plasmid

#82220

Purpose

Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PAK1 (PAK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DNASE2_WT

Plasmid

#81815

Purpose

Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNASE2 (DNASE2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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