We narrowed to 15,769 results for: LEA

1. pEGFP-N1-GCaMP6m-Xn

   Plasmid

   #118976

   Purpose

   Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

   Depositor

   Xiaodong Liu

   Article

   Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

   Insert

   GCaMP6m-XN
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Expression

   Bacterial

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 Lifeact-EGFP-2XNLS

   Plasmid

   #58467

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Lifeact
   2X NLS with 2X stop codons
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mCherry-LKB1(WT)

   Plasmid

   #84639

   Purpose

   mCherry-tagged, wild-type LKB1

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1 (STK11 Synthetic, Human)
   

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Cyto-GCaMP3

   Plasmid

   #64853

   Purpose

   Cytosol-targeted version of the Ca2+ indicator GCaMP3.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   GCaMP3
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP943

   Plasmid

   #104157

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fab specific nanobody TP886
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDB364

   Plasmid

   #110275

   Purpose

   N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   10-His, TEV cleavage peptide, and mature DsbC (i.…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-NES-ZapCV2-Zinc dead

   Plasmid

   #203850

   Purpose

   Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2-Ala zinc dead sensor
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Cys and His in Zap1 is mutated with Ala

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGCDNsam-Hnf4a-IRES-GFP

    Plasmid

    #33006

    Depositor

    Atsushi Suzuki

    Article

    Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

    Insert

    hepatic nuclear factor 4 alpha (Hnf4a Mouse)
    

    Use

    Retroviral

    Available Since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ExRai-CKAR2

    Plasmid

    #236096

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid

    #223245

    Purpose

    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry

    Depositor

    Stavros Lomvardas

    Insert

    Mex3a (Mex3a Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    4 point mutations in RING Domain: Cys 484 Ala; Hi…

    Promoter

    CMV

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBG100-TCPTP

    Plasmid

    #33365

    Depositor

    Ambra Pozzi

    Article

    Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

    Insert

    TCPTP-45 (PTPN2 Human)
    

    Tags

    6xHIS - cleavable

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEmCherry2

    Plasmid

    #118413

    Purpose

    Red fluorescent reporter for assessing CRISPR gRNA activity

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSIIpuro2-H2B-iRFP

    Plasmid

    #197352

    Purpose

    Encoding nuclear-targeted iRFP713.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    iRFP with nucleus-targeting sequence
    

    Use

    Lentiviral

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ABCB10-ChR2-eYFP

    Plasmid

    #206037

    Purpose

    Express ChR2 in mitochondria

    Depositor

    Lufang Zhou

    Article

    Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

    Insert

    ChR2
    

    Tags

    ABCB10 mitochondrial leading sequence and EYFP

    Expression

    Mammalian

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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