We narrowed to 15,888 results for: LEA
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Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…MutationRemoved stop codon to fuse it to the 2A-eGFPnls i…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2a wt
Plasmid#45445DepositorInsertNR2A (Grin2a Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL7044
Plasmid#167215Purposeoverexpression of PbrR in E. coli for cell-free lead sensingDepositorInsertPbrR
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7061
Plasmid#167230Purposecell-free cascaded lead sensorDepositorInsertpPbr-AKSIRV
UseSynthetic BiologyTags6X HisAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-RFP
Plasmid#221813PurposeLentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-mCherry
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneTagsHA-Twin-StrepExpressionPlantPromoter2 X 35SAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMEH303 NLS dTomato 6x high affinity MS2 loops
Plasmid#168215PurposeMammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-GCaMP6m-Xn
Plasmid#118976PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleusDepositorInsertGCaMP6m-XN
ExpressionMammalianAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-hph
Plasmid#117232PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-EPSIN 1
Plasmid#22228DepositorInsertEpsin 1 (Epn1 Rat)
TagsGFPExpressionBacterial and MammalianMutationplease see depositor comment belowAvailable SinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
ExpressionYeastMutationL477V, L640Y, I777K, W814NAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR1 wt
Plasmid#45446DepositorInsertNR1 (Grin1 Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCD-GSDMA-N-tev-C
Plasmid#226231PurposeExpress TEVP-cleavable GSDMADepositorInsertGasdermin A (Gsdma3 Mouse)
ExpressionMammalianAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only