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pSSa23

Plasmid

#177373

Purpose

Expresses red-shifted luciferase under TEF1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

TEF1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0134

Plasmid

#162307

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSAD-F3-NPEST-mCherry

Plasmid

#104332

Purpose

Self-inactivating DG-Rabies (SiR) expressing mCherry

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Insert

mCherry

Use

Unspecified

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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FtsZ

Plasmid

#74128

Purpose

Expresses FtsZ without a peptide

Depositor

Lynne Regan

Insert

FtsZ

Tags

C-terminal linker sequence (G G S G S G S S)

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCri-15b

Plasmid

#61336

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Yeast

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PGTE+mU6-WT

Plasmid

#12116

Depositor

Inder Verma

Article

Tiscornia et al Proc Natl Acad Sci U S A. 2004 May 11. 1

Insert

mU6 promoter

Expression

Bacterial

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 human A3F-CTD

Plasmid

#105052

Purpose

Overexpression of APOBEC3F C-terminal domain in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3F (A3F) C-terminal domain (APOBEC3F Human)

Tags

HA tag

Expression

Mammalian

Mutation

it contains only C terminal domain of A3F

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAM-35s-CERK1 D441V-YFPc-1

Plasmid

#102403

Purpose

split YFP. Plant expression of CERK1 D441V-YFPc-1

Depositor

Silke Robatzek

Insert

CERK1 (CERK1 Mustard Weed)

Tags

YFPc

Expression

Plant

Mutation

D441V

Promoter

35S

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSV CDK9 (D167N) HA (P#601)

Plasmid

#14639

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHygCG2r

Plasmid

#200074

Purpose

hygR selection mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHygRR2

Plasmid

#200075

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHygR2r

Plasmid

#200072

Purpose

hygR selection nls-mScarlet marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMCSGC-GB1

Plasmid

#186786

Purpose

E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8_MF (VE5596)

Plasmid

#139768

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Type

Empty backbone

Promoter

PH or p10

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT7RNAP-Q737P

Plasmid

#175356

Purpose

Support plasmid for T7_ori plasmids

Depositor

Sven Panke

Article

Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

Insert

T7RNAP-Q737P

Expression

Bacterial

Mutation

Q737P

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pELxG4

Plasmid

#172570

Purpose

Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

Depositor

Christopher Reisch

Insert

LuxR-GFPmut3

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pACyR1

Plasmid

#172565

Purpose

Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.

Depositor

Christopher Reisch

Insert

CymRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCA1.332 pDUOTK1-L1

Plasmid

#162351

Purpose

Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

Depositor

Alistair McCormick

Article

Gale et al Front Microbiol. 2021 Jan 15;11:624011.

Inserts

eYFP
LacZ alpha
mTagBFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Auxiliary A

Plasmid

#153352

Purpose

Provides End-to-End linker 4A for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4A

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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