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pcDNA-MYC-As-NF-kB1

Plasmid

#180203

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII-AGOhook_F

Plasmid

#146299

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII-AGOhook (TNRC6A Human)

Expression

Mammalian

Mutation

Four non silent mutations A339T, R934G, D1289G, a…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-Cterm

Plasmid

#180208

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 542-1224

Promoter

AdhI

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NMI

Plasmid

#178446

Purpose

doxycycline-inducible expression of Human NMI in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

NMI (NMI Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BATF2

Plasmid

#178453

Purpose

doxycycline-inducible expression of Human BATF2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF2 (BATF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB3

Plasmid

#180211

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-412

Promoter

AdhI

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB3

Plasmid

#180202

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-412

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB-Cterm

Plasmid

#180199

Purpose

Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kBCterm

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 542-1224

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mRuby3-DDX21 HGV

Plasmid

#179233

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

Plasmid

#175586

Purpose

in vitro transcription of mut RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz mut 2598-4066

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dBR

Plasmid

#176046

Purpose

Expresses Myc Mutant (delta basic region) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 355-369

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC002

Plasmid

#171139

Purpose

For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J1-BBa_J23117-sfGFP
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

J1-BBa_J23117 and Sp.pCas9, BBa_J23107

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-125b-2

Plasmid

#169311

Purpose

Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA3

Plasmid

#171001

Purpose

HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIV]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDwt

Plasmid

#167119

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3486 (YCp LEU2 Rpb1 F1492A)

Plasmid

#91808

Purpose

Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed phenylalanine 1492 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3487 (YCp LEU2 Rpb1 S1493A)

Plasmid

#91809

Purpose

Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed serine 1493 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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