Skip to main content
Addgene
Showing: 2241 - 2280 of 2924 results

  5. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    153551-plasmid-map-sequence-id-326384

    Enlarge

  6. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

    Enlarge

  15. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

    Enlarge

  16. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

    Enlarge

  18. pUb-tdTomato-LP-Neo-LP

    Plasmid
    #231509
    Purpose
    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system
    Depositor
    Yeonchul Hong
    Insert
    tdTomato fluorescent protein
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    Expression
    Mutation
    Promoter
    Acanthamoeba polyubiquitin
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231509-plasmid-map-sequence-id-462659

    Enlarge

  24. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid
    #182532
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    hM4Di-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182532-plasmid-map-sequence-id-361681

    Enlarge

  29. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183766-plasmid-map-sequence-id-361683

    Enlarge

  30. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

    Enlarge

  32. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

    Enlarge

  35. pAAV-TRE-DIO-GCaMP6s

    Plasmid
    #183809
    Purpose
    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene TetO promoter-DIO/flex-GCaMP6
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183809-plasmid-map-sequence-id-366066

    Enlarge

  39. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183765-plasmid-map-sequence-id-361682

    Enlarge

  40. pJW1586

    Plasmid
    #121057
    Purpose
    mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2-T^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized mKate2, and au…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121057-plasmid-map-sequence-id-235972

    Enlarge

Showing: 2241 - 2280 of 2924 results