We narrowed to 7,692 results for: Lif;
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Plasmid#123354PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.DepositorInsertcp-moxCerulean3-moxCerulean3-FLARE-D1ER
TagsC-terminal ER retention signal (KDEL), cp-moxCeru…ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-CKAR
Plasmid#123345PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.DepositorInsertmCherry-mCherry-FLARE-CKAR
Tags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR
Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [dRING]
Plasmid#126549PurposeExpresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationRING domain deletion mutant [dRING mutation is a …PromoterLTRAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [Y252D]
Plasmid#126550PurposeExpresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationY252D point mutantPromoterLTRAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]
Plasmid#124068PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxExpressionInsectPromoterbhsp68Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-Fc-His
Plasmid#72189PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.26
TagsFc-HisExpressionMammalianPromoterCMVAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-Fc-His
Plasmid#72187PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.23
TagsFc-HisExpressionMammalianPromoterCMVAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-AP-His
Plasmid#72063PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.26
TagsAP-HisExpressionMammalianPromoterCMVAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2B-siRNAres
Plasmid#115333Purposeexpresses siRNA-resistant mCherry-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationmutated to be resistant to siRNA knockdownPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321D
Plasmid#89288PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AspPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325D
Plasmid#89289PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AspPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 E322A
Plasmid#87748PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Glu-322 to AlaPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325A
Plasmid#87749PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AlaPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321A
Plasmid#87747PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AlaPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP-DCRD
Plasmid#87453Purposerapamycin-induced membrane-targeting of DCRDDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, FKBP, and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP-PCRD
Plasmid#87452Purposerapamycin-induced membrane-targeting of PCRDDepositorInsertPCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
TagsFKBP and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS K283D Q287D
Plasmid#79068PurposeExpress owl monkey TRIMCyp K283D, Q287D in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
TagsOneSTrEP-FLAGExpressionMammalianMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterCMVAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(S)-Fc-His
Plasmid#72127PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.6-Fc-His
Plasmid#72103PurposeExpresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.2-Fc-His
Plasmid#72099PurposeExpresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.e-Fc-His
Plasmid#72114PurposeExpresses the extracellular region of the Netrin G1, isoform e protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.g-Fc-His
Plasmid#72116PurposeExpresses the extracellular region of the Netrin G1, isoform g protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.d-Fc-His
Plasmid#72113PurposeExpresses the extracellular region of the Netrin G1, isoform d protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-Fc-His
Plasmid#72121PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,-)-Fc-His
Plasmid#72148PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(L)-Fc-His
Plasmid#72139PurposeExpresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(S,-)-Fc-His
Plasmid#72150PurposeExpresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,-)-AP-His
Plasmid#72022PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(S,-)-AP-His
Plasmid#72024PurposeExpresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.g-AP-His
Plasmid#71990PurposeExpresses the extracellular region of the Netrin G1, isoform g protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(S, +)-Fc-His
Plasmid#72149PurposeExpresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(S)-Fc-His
Plasmid#72140PurposeExpresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.30 EC10-AP-His
Plasmid#72064PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.30
TagsAP-HisExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-AP-His
Plasmid#72060PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.22
TagsAP-HisExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-AP-His
Plasmid#72061PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.23
TagsAP-HisExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.f-Fc-His
Plasmid#72094PurposeExpresses the extracellular region of the Neogenin 1, isoform f protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.c-AP-His
Plasmid#71986PurposeExpresses the extracellular region of the Netrin G1, isoform c protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.f-AP-His
Plasmid#71989PurposeExpresses the extracellular region of the Netrin G1, isoform f protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.b-AP-His
Plasmid#71985PurposeExpresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.2-AP-His
Plasmid#71973PurposeExpresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(S)-AP-His
Plasmid#72001PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.h-AP-His
Plasmid#71991PurposeExpresses the extracellular region of the Netrin G1, isoform h protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-Fc-His
Plasmid#72186PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.22
TagsFc-HisExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.e-AP-His
Plasmid#71988PurposeExpresses the extracellular region of the Netrin G1, isoform e protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.6-AP-His
Plasmid#71977PurposeExpresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(S)-AP-His
Plasmid#72014PurposeExpresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.5-AP-His
Plasmid#71976PurposeExpresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.4-AP-His
Plasmid#71975PurposeExpresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only